Single Cell Europe Conference

September 19 - 21, 2018

Workshop Wednesday, September 19
Single cell isolation
RT-qPCR Single cell expression profiling
microRNA analysis
15:4516:40CONFERENCE REGISTRATION, Poster setup
16:4016:50Chairman TALK Mikael Kubista / Conference hall
16:5017:30Plenary Speaker / Anders Ståhlberg / Tumor heterogeneity and liquid biopsies: The use of single-cell and single-molecule analyses in breast cancer and liposarcoma
17:3018:00Siegfried Hauch An easy method for CTC single cell isolation and subsequent molecular profiling
18:00BEER EVENT with music
Conference Thursday, September 20
08:0009:00REGISTRATION, poster setup
09:0009:10WELCOME - Pavel Martásek, BIOCEV director
Session 1Chairman: 1 Mikael Kubista
09:1009:50Marc Wadsworth II Approaching the Immune System as an Interacting Ensemble of Cells
09:5010:30Martin Hemberg Computational tools for analyzing large collections of scRNA-seq datasets
10:3010:4510x GENOMICS presentation
Session 2Chairman: 2
11:2011:40Catherine Alix-Panabières Molecular and functional characterization of circulating tumor cells in carcinoma patients
11:4012:00Lukáš Valihrach Single cell analysis of oligodendroglial lineage cells during post-ischemic regeneration
12:0012:20Evi Lianidou Liquid Biopsy: The clinical potential of single Circulating Tumor Cell analysis
12:2012:40Leon Terstappen Tools to identify and characterize single Circulating Tumor Cells
12:4013:00Nikolas Stoecklein Molecular Characterization of CTCs detected with the CellSearch System
13:0013:20Katarína Kološtová Character of circulating tumor cells as a part of liquid dynamic medicine in oncology
Session 3Chairman: 3
15:2016:00Fuchou Tang Deciphering the gene regulation networks in human early embryos
16:0016:40Norman Dovichi Proteomics of early development: the assembly of the vertebrate brain
16:4017:00Radek Šindelka Subcellular transcriptome and proteome analyses to reveal body plan determinants of early development
17:0017:10Samuel Bouet - SMARTer Solutions for Single-Cell Transcriptomics and Genomics
Conference Friday, September 21
08:0009:00poster setup
Session 4Chairman: 4
09:0009:40Vessela N. Kristensen Tracing the origin of disseminated tumor cells in breast cancer using single-cell sequencing
09:4010:00Pamela Pinzani Molecular analysis of single circulating tumor cells from cancer patiens
10:0010:20Mikael Kubista Two Tailed-PCR for precision diagnostics
10:2010:40Christer Ericsson Screening circulating tumor cells as a non-invasive cancer test in 3,388 individuals from high-risk groups (ICELLATE2)
10:4011:00Guilhem Tourniaire - cellenONE X1 : A versatile platform for single cell isolation
11:0011:15DOLOMITE presentation
Session 5Chairman: 5
11:4512:25Pascal Barbry A new functional genomics approach to understand the human airways at a single cell level
12:2512:45Ilaria Santeramo Single cell Viral Copy Number analysis using ddPCR
12:4513:05Peter Karlberg Celsee Genesis: Comprehensive Single Cell Preparation
13:0513:25Larry Xi Single Cell DNA Sequencing - transition from analog mode to digital mode
13:2513:55Jennifer Chew - SureCell ATAC-Seq: A new platform for Single Cell ATAC-seq using Bio-Rad’s droplet digital technology
Session 6Chairman: 6
15:2016:00Michael W. Pfaffl Exosomal microRNA Biomarkers Signatures in Clinical Diagnostics
16:0016:20Pavel Vodička Search for prognostic and predictive markers in sporadic CRC
by Mikael Kubista
WORKSHOP September 19, 2018

Single cell isolation

Learn how to isolate single cells from tissue and suspension!

Target audience: Researchers interested in isolating single cells.

Description: This workshop provides an introduction into advanced technologies for the selective and precise isolation of single cells. Laser microdissection can be applied to isolate cells from tissue as well as from live cell culture. A microcapillary-based method is employed to isolate cells from suspension, such as CTCs from liquid biopsies. This workshop offers the following:

  • Introduction to single cell isolation technologies
  • Scientific presentation from users
  • Applications
  • Tips and tricks for sample preparation and downstream analyses
  • Hands-on training

Single cell analysis

Learn how to explore heterogeneity within cell population, find cell subpopulations, gene correlations using single-cell analysis, how to interpret and apply single-cell data in biological studies

Target audience: Medium to Advanced qPCR users

Entrance qualifications: Hands-on qPCR TATAA course or similar knowledge

Description: In this course you will learn to recognize the difference between cell population and single-cell analysis and how to interpret and apply single-cell data in biological studies. Practical training in cell collection, gene expression profiling and data analysis at the single-cell level is included. The course contains:

  • Overview of single-cell biology: methods, examples and trends
  • Cell population versus single-cell approach
  • Cell collection methods, dicsussion about FACS, automated cell picking CellCelector
  • Gene expression profiling workflow RT, qPCR, (NGS), quality control
  • Single-cell data analysis, including practical examples (Genex)

microRNA analysis

Date: September 19, 2018, 9 am - 5:30 pm
After 5:30 pm Beer event in Biocev campus

Learn how to extract and analyze miRNA with appropriate technique!

Target audience: Researchers interested in analyzing microRNA.

Entrance qualifications: Basic knowledge about microRNA and molecular analyses.

Description: This course introduces miRNA including its variant forms. Methods to extract and purify microRNA and prepare it for analysis including quality control. Methods for microRNA analysis are presented and compared, to learn how to choose the best method for each study. - Analysis of microRNA data is also discussed including challenging involving normalization. In the course you will learn:

  • microRNA background
  • miRNA sampling, extraction and quality control
  • microRNA analysis with microarray, NGS, RT-qPCR methods
  • Data analysis and interpretation
  • Liquid biopsies
  • Introduction to functional studies

May 31, 2018

Early Registration Deadline

March 31, 2018

Oral presentation submissions deadline

May 31, 2018

Poster submissions deadline